Bari and Bologna have been knocked out of the Coppa Italia at the third round stage by second division opponents, but Atalanta, Catania, Chievo and Palermo all progressed.
The biggest casualty of the night took place in Ravenna where Bologna went out on penalties against Serie B outfit Frosinone.
With no goals separating the teams inside 90 minutes and extra time, the match was decided on penalties with Frosinone goalkeeper Vincenzo Sicignano saving two penalties to record a 7-6 win.
Despite Frosinone missing their first spot kick through Gennaro Scarlato, Sicignano was the hero saving Bologna's second effort from Miguel Britos and their eighth from Cristian Zenoni, giving Migjen Basha the opportunity to slot in the winning strike.
The Canarini will now face Chievo in the fourth round. The Flying Donkeys eased past Mantova with a 3-0 win thanks to goals from Erjon Bogdani, Sergio Pellissier and Simone Bentivoglio.
Newly-promoted Bari were eliminated after a penalty shoot-out against Empoli at the Stadio San Nicola.
It looked as though Empoli had secured the win against their top flight opponents inside the 90 minutes when Francesco Lodi gave the visitors the lead with 14 minutes to go. However, the hosts equalised six minutes later through Giuseppe Greco to force extra time and penalties.
Defender Andrea Masiello missed the only penalty of the shoot-out as Salvatore Campilongo's Empoli won 5-4 to set up a fourth round tie with Catania, who struggled to a 1-0 win over third tier Cremonese thanks to Giuseppe Mascara's strike.
Sicilian rivals Palermo also had to deal with Lega Pro 1 opposition, beating Spal 4-2 at the Stadio Renzo Barbera.
Walter Zenga's side were given a massive scare when Gianluca Laurenti gave the visitors a shock lead after 21 minutes. However, Fabrizio Miccoli turned the game around with a brace, his first a penalty, before the interval.
Fabio Simplicio and Edison Cavani extended Palermo's lead in the second half while Spal got a late consolation through Rachid Arma.
Palermo will now meet Reggina after the recently-relegated side recorded an impressive 1-0 win at Serie B promotion hopefuls Brescia with Franco Brienza scoring the winner.
Following Novara's progress after their shock 2-1 win at Parma on Friday night, Lumezzane will be the second Lega Pro 1 club in the fourth round of this season's Coppa Italia.
The minnows from Lombardia embarrassed Ancona 3-2, taking the lead twice through Massimiliano Pesenti and Fabio Lauria. Federico Gerardi and Salvatore Mastronunzio replied to level the scores for Ancona but they had no answer to Michele Marconi's winning goal.
Lumezzane's fourth round opponents will be Atalanta after the Nerazzurri squeezed their way past Cesena.
Paolo Bianco's sending off made the task difficult for Angelo Gregucci's side but they were thankful to new signing Robert Acquafresca, who scored the winning goal six minutes from time.
Cittadella needed extra time to come from behind and defeat fellow second division outfit Ascoli 2-1.
The visitors took the lead through Simone Pesce, but Leonardo Pettinari levelled to force extra time. Matteo Ardemagni secured victory with only minutes left to avoid the dreaded penalty shoot out.
The rest of the third round matches, with the exception of Siena-Grosseto, will be completed on Sunday night.
Third Round Results
Friday 14 August 2009
Sassuolo 2-0 Verona
Parma 1-2 Novara
Livorno 2-0 Torino
Saturday 15 August 2009
Cittadella 2-1 Ascoli (AET)
Brescia 0-1 Reggina
Catania 1-0 Cremonese
Palermo 4-2 Spal
Ancona 2-3 Lumezzane
Bari 1-1 Empoli (AET, Pens: 4-5)
Bologna 0-0 Frosinone (AET, Pens: 6-7, Played in Ravenna)
Cesena 0-1 Atalanta
Chievo 3-0 Mantova
Third Round Fixtures
Sunday 16 August 2009
Triestina v Cagliari (1900)
Napoli v Salernitana (1930)
Sampdoria v Lecce (1930)
Thursday 12 November 2009
Siena v Grosseto (1400)
* All kick-off times listed are UK time (+1 for Italy/CET). Times, dates and fixtures are subject to change.
Coppa Italia Third Round Reviews
[14 Aug 2009] - [15 Aug 2009] - [16 Aug 2009] - [12 Nov 2009]
Source: Lega Calcio