Just weeks after their relegation from Serie B, Avellino, Pisa and Treviso, along with Venezia, have been thrown out of the professional football leagues.
All clubs in Italy must prove their financial stability to Covisoc before they are allowed to register for the new season. However, it was announced that a total of sixteen clubs from the third and fourth tiers of Italian football failed the inspections.
The failure to provide further financial documents by Saturday's deadline had resulted in four clubs being declared bankrupt and excluded from the next Lega Pro Prima Divisione season.
Sambenedettese, of Lega Pro Seconda Divisione, also decided not to appeal, while fourth tier rivals Ivrea and newly-promoted Biellese had already thrown in the towel when Covisoc reported their investigations last week.
Three clubs did decide to appeal with Perugia of Lega Pro 1 and Catanzaro of Lega Pro 2 both successful at today's FIGC Federal Council meeting in Rome but fourth tier outfit Pistoiese were rejected and are the eighth club thrown out.
"It is always painful to lose clubs, in this case some very glorious ones," said Lega Pro President Mario Macalli. "I can say that if the clubs coming down from Serie B had remained in that division, they would have continued playing even with 10million euros of debt.
"It is different for us in Lega Pro, where even 100,000 euros is enough to send you under."
Lega Pro 2 clubs Alghero, Barletta, Igea Virtus, Legnano, Pro Sesto and Vibonese - all of whom initially failed to comply with the Covisoc committee, are now in the clear and will be allowed to register for the 2009-10 season, which is set to begin on the weekend of August 23.
Pisa had a dramatic final round of the Serie B season as a flurry of late goals condemned the Tuscans to relegation back to the third tier. But debts of reportedly around 7million euros meant the club, who went out of business in 1994, had to file for bankruptcy again.
Avellino, who finished second-from-bottom in Serie B last term, and Treviso, who finished last, also decided not to lodge appeals against their exclusions. The latter, in their centenary, were in Serie A just four years ago.
Since relegation from Serie B in 2005, Venezia have had to file for bankruptcy for the second time. The Venetians have been struggling in Lega Pro 1 for the last three years and only survived in the third tier last term via a play-out tie.
Most of these clubs will be expected to apply for a place in the regional amateur Serie D league - the highest non-professional league, next season.
The four clubs that will be "promoted" to Lega Pro 1 and the eight "new" teams that will be entered into Lega Pro 2 will be announced at the Federal Council's next meeting on July 30.