New Italy coach Cesare Prandelli has named Antonio Cassano, Mario Balotelli and Amauri in his squad for his first game in charge against Ivory Coast in a friendly on Tuesday.
Internazionale's 19-year-old striker Balotelli, being courted by Manchester City despite a series of on-and-off the field problems, earns his first call-up alongside Brazil-born forward Amauri.
The inconsistent Juventus striker had the chance of making the Brazil squad last year but hung on to gain Italian citizenship only to be ignored by Marcello Lippi for Italy's World Cup humiliation in June.
In all there are eight new call-ups and only nine players remain from the squad which went to South Africa as defending champions but were dumped out in the group stage without winning a game.
Fabio Cannavaro and Gennaro Gattuso retired from internationals after the debacle and former Fiorentina boss Prandelli has signalled a possible end to the Italy careers of Antonio Di Natale and Gianluca Zambrotta by leaving them out.
Gianluigi Buffon and Andrea Pirlo are not fit so Roma's Daniele De Rossi is set to be captain for the game at West Ham United's Upton Park Stadium in London.
Sampdoria talisman Cassano, who has overcome his bad-boy image in recent seasons, was also overlooked by Lippi for two years but makes his return to the squad having last been involved at Euro 2008.
Alberto Gilardino of Fiorentina and Sampdoria's Giampaolo Pazzini, players who Prandelli has worked with in the past, haven't been selected.
There are new and internationally inexperienced faces in the squad with goalkeeper Emiliano Viviano and defenders Luca Antonini, Davide Astori, Mattia Cassani, Stefano Lucchini, Cristian Molinaro and Marco Motta .
Italy start their Euro 2012 qualifying campaign in Estonia on September 3 before hosting the Faroe Islands in Florence four days later.
The squad will gather on Sunday at the FIGC's Technical centre in Coverciano, before their flight to London on Monday.
Goalkeepers: Federico Marchetti (Cagliari), Salvatore Sirigu (Palermo), Emiliano Viviano (Bologna).
Defenders: Luca Antonini (Milan), Davide Astori (Cagliari), Leonardo Bonucci (Juventus), Mattia Cassani (Palermo), Giorgio Chiellini (Juventus), Stefano Lucchini (Sampdoria), Cristian Molinaro (Stuttgart), Marco Motta (Juventus).
Midfielders: Daniele De Rossi (Roma), Andrea Lazzari (Cagliari), Claudio Marchisio (Juventus), Ricardo Montolivo (Fiorentina), Angelo Palombo (Sampdoria), Simone Pepe (Juventus).
Forwards: Amauri (Juventus), Mario Balotelli (Inter), Marco Borriello (Milan), Antonio Cassano (Sampdoria), Fabio Quagliarella (Napoli), Giuseppe Rossi (Villarreal).