The stadium in Bari has been declared fit to stage the Euro 2008 qualifier between Italy and Scotland on March 28.
It had been one of the 25 venues closed to spectators by security chiefs in the crackdown that followed the death of a police officer.
The Stadio San Nicola and Parma's Stadio Ennio Tardini have had their stadiums re-opened after the arenas complied with the Legge Pisanu safety regulations and fans will be allowed entry from this weekend. The Prefect of Police Antonio Manganelli said: "The stadia that meet the norms are Bari and Parma. They will be open to everyone from this coming Sunday."
Stadium work at Bologna and Reggina continue, but the two local clubs have been given clearance to allow season ticket holders entry for the time being.
But the decision would appear to clear the Italy versus Scotland match to be played at the stadium, which was designed by famous architect Renzo Piano for the 1990 World Cup.
It also staged the 1991 European Cup final between Red Star Belgrade and Marseille.
The visitors will receive at least 5,800 tickets if the game goes ahead as expected at full capacity in Bari. But the Scottish Football Association is still awaiting confirmation that the clash will go ahead as planned.